Separation and Purification of Sovateltide Using NanoPak-C All Carbon Microbeads

We have released an application note that provides a strong and scalable approach for the purification

Figure. Chemical structure depiction of Sovatelltide. Source. PubChem CID 16130933.

and detailed characterization of Sovateltide, a peptide drug that is currently attracting considerable interest in the medical research field. Sovateltide is approved for treating acute ischemic stroke (AIS) in India and is undergoing phase III clinical trials for approval in the United States. AIS occurs when blood flow suddenly stops to a part of the brain, typically affecting a specific area supplied by a blood vessel.

Sovateltide is a synthetic analog of the naturally occurring peptide endothelin-1 and acts as an endothelin-B receptor agonist. This type of agonist activates the endothelin-B receptor, which is found on certain cells in the body, including astrocytes in the brain. Astrocytes, also known as astroglia, represent the largest and most abundant category of glial cells in the Central Nervous System (CNS). These are supportive “glue” cells that provide a structure to support and insulate neurons. They cannot conduct electrical impulses.

When the agonist binds to this receptor, it triggers cellular responses, such as regulating cell growth, especially in the context of brain injury. In simpler terms, you can think of the endothelin-B receptor as a lock on a cell and the agonist as a key that fits into this lock, unlocking it to initiate specific actions within the cell. These actions can aid in processes such as healing following a brain injury.

Sovateltide is typically purified using reverse-phase high-performance liquid chromatography (HPLC) under basic conditions, whereas its characterization is performed under acidic conditions. This combined method enables the isolation of the pure compound, as well as the determination of optimal analytical conditions for confirming its identity, purity, and structure.

Our dual-method approach, employing NanoPak-C All Carbon HPLC columns, provides a robust and scalable solution for both the purification and comprehensive characterization of complex peptides, such as Sovateltide and similar endothelin-1 analogs.

Download our application note here.

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