A validated RP-HPLC method for semaglutide and liraglutide on NanoPak-C all-carbon microbeads demonstrates R² > 0.99 linearity, LOD as low as 0.0024 mg/mL, and system precision below 2% %RSD — all at pH 8.5. We explain what each validation parameter means for QC labs, why liraglutide data extends the platform, and how this ICH Q2(R1)-compliant method is ready for regulatory submissions and multi-site method transfer.

Proteomics labs increasingly push LC–MS systems to their limits, yet sample prep is often constrained by media that are suboptimal for today’s harsh conditions, complex matrices, or high-sensitivity PTM analysis All‑carbon microbeads with tunable pore size and surface chemistry offer a way to decouple capacity from “stickiness” so you can match the bead to the biology, not the other way around.

Crude GLP-1–class peptides and other long sequences typically enter purification at 45–65% area purity. The dominant impurities present...

We have released an application note that provides a strong and scalable approach for the purification Figure. Chemical structure...

This blog provides an overview of column cleaning and regeneration techniques to extend the lifetime of reverse-phase HPLC columns.

This blog, part of our two-part series on extending the lifetime of HPLC Columns, explores the probable causes of column contamination.

A pharmaceutical's purity, assay, and potency are terms commonly used when discussing drug quality testing. Due to its accuracy and...

This blog delves into the selectivity differences between our NanoPak-C all-carbon graphitic stationary phase and other media.

Millennial Scientific is pleased to announce a major achievement in our product development: the successful completion of the beta...

Flash chromatography is a valuable tool in peptide purification workflows, especially as a pre-purification or cleanup step.

This blog provides an easy-to-understand overview of hydrophobic interaction chromatography. Part of our separation science primer series.

This post is part of our separation science primer series which provides an easy-to-understand overview of key topics in Flash...

NanoPak-C All Carbon media has adjustable pore size and surface area that is suitable for separating proteins in reverse phase. We demonstra

We have published an application note that presents a reliable and quick method using NanoPak- C All carbon columns for simultaneously...

In our previous blog, we explained the differences between NanoPak-C All Carbon microbeads and alkyl bonded silica . In this blog, we...

We have published an application note that presents an optimized method for HPLC separation of semaglutide and liraglutide. This method...

The constant monitoring of pesticides is imperative due to their potential environmental harm. These chemicals, including insecticides,...

Oligonucleotides are tiny fragments of DNA or RNA. Our genetic information is stored in DNA and RNA. These molecules are also known as...

Workflow for NanoPak-C All Carbon SPE of Trace Organochlorine Pesticides from Small Volume Complex Biological Samples

The Basics The most widely used mode of chromatography is Reversed-Phase Liquid Chromatography (RPLC).  Accounting for more than 90% of...