Bridging analytical and preparative HPLC, this blog shows how NanoPak‑C all‑carbon columns withstand tens of thousands of column volumes at pH 8.5 in GLP‑1 methods. By expressing alkaline-pH exposure in column volumes, we model NanoPak‑C stability for scale-up from analytical to semi‑prep and prep purification of Semaglutide, Liraglutide, and other peptide drugs.

NanoPak-C All-Carbon capillary HPLC columns offer porous graphitic carbon selectivity for glycans, glycopeptides, PTM-rich peptides, and polar metabolites in nanoLC–MS proteomics workflows. In an independent head-to-head comparison with commercial PGC and C18, NanoPak-C showed sharper peaks, faster column stabilization, and a cleaner MS baseline. Read our technical note and blog to learn where all-carbon media fits in bottom-up proteomics.

This blog delves into the selectivity differences between our NanoPak-C all-carbon graphitic stationary phase and other media.