Millennial Scientific will present a poster and exhibit our products and services at the Eastern Analytical Symposium & Exposition 2023.
Every year, the Eastern Analytical Symposium and Exposition (EAS) brings professional scientists and students together and provides continuing education in the analytical and allied sciences through symposia of papers, workshops, and short courses. An exposition of analytical science companies is held concurrently with the symposia.
Our poster abstract details are below.
Poster abstract :
All Carbon Stationary Phase Material for Biomolecule Separation: Design and Characterization
Michael Jack Parente, Balaji Sitharaman
Millennial Scientific, 25 Health Sciences Dr., Suite 123, Stony Brook, NY 11790
Synthetic graphitic carbon and bonded silica were liquid chromatography's earliest-studied reverse-phase media.1 Due to material and manufacturing innovations, along with the early timeline of its introduction, bonded silica-based media dominates reverse-phase separations.
Figure 1: Depiction of the material synthesis method. A viscous suspension containing a mixture of carbon material and crosslinker is passed through a microfluidic device to generate uniform-sized droplets. UV or thermal energy facilitates in situ binding, generating mechanically stable spheres. Collected microbeads are annealed at high temperatures in a rotary drum under a vacuum to remove the unreacted components to purify the microbeads. The key parameters that control the size of the microbeads are the size of the capillary tube and outer phase viscosity and flow velocity.
Despite the early promise of porous graphitic carbon, there have been limited efforts in developing novel carbon-based reverse stationary phase media.
This presentation highlights recent advancements in carbon-based microbead stationary phase media synthesized using carbon nanomaterials (carbon nanotubes, graphene, fullerenes) and micrographite as feedstock.
First, an overview of the material synthesis platform will be presented, describing its unique capabilities. A scalable microfluidic-based platform technology, specifically designed to pump highly viscous materials (Figure 1), enables manufacturing outside the purview of traditional microfluidic-based processes. The viscosities of materials processed by our state-of-the-art setup are between 300-600 mPa.s., which is hundreds of times greater than materials processed in off-the-shelf microfluidic systems.
Figure 2:Representative images of carbon microbeads. (a) bright-field optical microscopy. (b) low and (c) high resolution scanning electron microscopy (SEM).
Next, using natural graphite carbon-based stationary phase media as an example, a summary of the fundamental physicochemical properties (Figure 2 and Table 1) of the microbeads will be presented.2
Figure 3: Representative separation of recombinant DNA biomolecule insulin apart from its hydrocarbon preservatives phenol and metacresol using all carbon microbeads. Column: 50 x 2.1 mm, 7 µm, all carbon. Buffer: 0.2 M Sodium Sulphate, pH 2.3. Mobile phase A: 82/18 buffer/ACN, B:50/50 buffer/ACN. Flow 0.2 mL/min. Temp 35°C. Injection 1 µL. UV 214 nm.
Further, its differentiation over the current state-of-art reverse-phase chromatography media will be elucidated. Chromatography performance studies (Figure 3) will be discussed using several biomolecules as examples. Finally, future directions will be presented.
1. Ross, P. The Use of Porous Graphitic Carbon in Liquid Chrotnatography Perfonnance and Polar Retention Effect PhD thesis, University of Edinburgh, (1998).
2. Parente, M. J., Sitharaman, Balaji. Synthesis and Characterization of Carbon Microbeads. ACS Omega (2023).