A novel isoelectric focusing (IEF) platform for low‑input proteomics, using 3D‑printed immobilized pH gradient (IPG) carbon microbead arrays for first‑dimension prefractionation. NanoPak‑C‑IPG enables localized pI‑based protein focusing, high extraction efficiency, and direct hand‑off to all‑carbon NanoPak‑C RP‑HPLC, addressing key limitations of conventional IPG strips in single‑cell and low‑sample workflows.

Millennial Scientific is advancing NanoPak‑C, a 3D‑printed monolith platform designed to improve biologics chromatography. Supported by an NIH SBIR Phase I project and Empire State Development’s NYSTAR Innovation Matching Grants Program, this work helps bridge the gap between R&D and commercialization, enabling scalable, customizable separation media for more efficient bioprocessing.

NanoPak‑C is our new class of 3D‑printed carbon monoliths designed for miniaturized biologics chromatography. Built from proprietary all‑carbon microbeads, these microstructured devices retain a microwell‑plate format while enabling high‑performance solid‑phase extraction and chromatography of proteins and other complex biologics from ultra‑low‑volume samples.