HPLC Separation of Tirzepatide on NanoPak-C All Carbon HPLC columns

NanoPak‑C all‑carbon HPLC columns enable robust separation of tirzepatide under both acidic (pH 2.5) and alkaline (pH 8.5) conditions, with sharp peaks and predictable retention times at 5.3 and 6.8 minutes. This application note details the gradient, mobile phases, and system setup, and highlights how pH‑flexible methods support process development, purification, and QC workflows for GLP‑1–class peptides.