Capillary-HPLC Comparison of NanoPak-C All-Carbon, Porous Graphitic Carbon and C18 for NanoLC-MS of a Bovine Serum Albumin (BSA) Typtic Digest

This white paper presents an independent nanoLC–MS comparison of NanoPak‑C All‑Carbon microbead capillary columns with commercial PGC and C18 columns using an 80 fmol BSA tryptic digest, highlighting retention, efficiency, conditioning behavior, and MS baseline performance. It positions All‑Carbon media as a complementary, low‑bleed, fast‑conditioning option for hydrophilic peptides, glycans, glycopeptides, PTM‑rich peptides, and polar metabolites, while outlining a roadmap toward a broader suite of all‑carbon supports for proteomics separations.